Merlin (Phospho-S518) Rabbit Polyclonal Antibody

SKU: orb216168

Description

The Merlin (Phospho-S518) Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S518 of human Merlin protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: WB: 1:500-1:1000, IHC-P: 1:100-1-200
• Reactivity: Gallus, Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S518 of human Merlin protein. The exact sequence is proprietary.
• Target: NF2
• Molecular Weight: 70 kDa
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SCH; Merlin; Moesin-ezrin-radixin-like protein; Neurofibromin-2; Schwannomerlin; Schwannomin

Western blot analysis of Merlin (Phospho-S518) expression in Hela (A), A549 (B) whole cell lysates. (Predicted band size: 69 kD; Observed band size: 70 kD)

Immunofluorescent analysis of Merlin (Phospho-S518) staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Immunohistochemical analysis of paraffin-embedded mouse brain using Merlin (Phospho-S518) Rabbit Polyclonal Antibody orb216168 with dilution at 1:100.

Quick Database Links

Gene Symbol

NF2

UniProt

Q63648, P46662, P35240

NCBI Gene ID

18016, 25744, 4771