MBD4 Rabbit Polyclonal Antibody

SKU: orb99204

Description

Anti-MBD4 Antibody. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: WB
• Dilution Range: Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human MBD4, identical to the related rat and mouse sequences.
• Target: Methyl-CpG-binding domain protein 4
• Molecular Weight: 80 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Methyl-CpG-binding domain protein 4; 3.2.2.-; Methyl-CpG-binding endonuclease 1; Methyl-CpG-binding protein MBD4; Mismatch-specific DNA N-glycosylase; MBD4; MED1

Western blot analysis of Lane 1: Rat Brain Tissue Lysate, Lane 2: MCF-7 Cell Lysate, Lane 3: HELA Cell Lysate using MBD4 antibody

Western blot analysis of Lane 1: Recombinant Human MBD4 Protein 10ng, Lane 2: Recombinant Human MBD4 Protein 5ng, Lane 3: Recombinant Human MBD4 Protein 2.5ng using MBD4 antibody

Western blot analysis of Lane 1: Rat Brain Tissue Lysate, Lane 2: Rat Kidney Tissue Lysate, Lane 3: A549 Cell Lysate, Lane 4: HELA Cell Lysate, Lane 5: MCF-7 Cell Lysate using MBD4 antibody

Quick Database Links

Gene Symbol

Methyl-CpG-binding domain protein 4

UniProt

O95243