Lymphotactin/XCL1 Rabbit Polyclonal Antibody

SKU: orb315189

Description

Anti-Lymphotactin/XCL1 Antibody. Tested in ELISA, WB applications. This antibody reacts with Human.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: Western blot, 0.1-0.5μg/ml, Human ELISA (Cap), 1-5μg/ml
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Lymphotactin recombinant protein (Position: V22-G114). Human Lymphotactin shares 60.2% and 57% amino acid (aa) sequence identity with mouse and rat Lymphotactin, respectively.
• Target: Lymphotactin
• Molecular Weight: 13 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Lymphotactin; ATAC; C motif chemokine 1; Cytokine SCM-1; Lymphotaxin; SCM-1-alpha; Small-inducible cytokine C1; XC chemokine ligand 1; XCL1; LTN, SCYC1

Sandwich ELISA - Recombinant human Lymphotactin/XCL1 protein standard curve. Use in combination with reagents from Human Lymphotactin/XCL1 ELISA Kit EZ-Set (DIY Antibody Pairs).

Western blot analysis of Lymphotactin using anti-Lymphotactin antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: HELA Whole Cell Lysate at 40 ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lymphotactin antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Lymphotactin at approximately 13 kDa. The expected band size for Lymphotactin is at 13 kDa.

Quick Database Links

Gene Symbol

Lymphotactin

UniProt

P47992