LGALS3 Rabbit Polyclonal Antibody

SKU: orb1993150

Description

Anti-LGALS3 Antibody. Tested in WB, ICC/IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, ICC, IF, WB
• Dilution Range: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human ELISA, 0.1-0.5 μg/ml
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: E.coli-derived human Galectin-3/LGALS3 recombinant protein (Position: R129-I250). Human Galectin-3/LGALS3 shares 87.7% and 84.4% amino acid (aa) sequence identity with mouse and rat Galectin-3/LGALS3, respectively.
• Target: Galectin-3
• Molecular Weight: 29 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

35 kDa lectin; CBP 35; CBP35; Gal 3; GAL3; Galactose specific lectin 3; Galactoside binding protein; GALBP; Galectin 3; Galectin-3; GALIG; IgE binding protein; L 31; Laminin binding protein; Lectin L 29; LGALS2; LGALS3; Mac 2 antigen; MAC2

IF analysis of Galectin-3/LGALS3 using anti-Galectin-3/LGALS3 antibody. Galectin-3/LGALS3 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 µg/mL rabbit anti-Galectin-3/LGALS3 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Western blot analysis of Galectin-3/LGALS3 using anti-Galectin-3/LGALS3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat stomach tissue lysates, Lane 6: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Galectin-3/LGALS3 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Galectin-3/LGALS3 at approximately 29 kDa. The expected band size for Galectin-3/LGALS3 is at 26 kDa.

Quick Database Links

Gene Symbol

Galectin-3

UniProt

P17931