Anti-LAMP1 Antibody

• Catalog Number: orb76335
• Category: Antibodies
• Description: Anti-LAMP1 Antibody. Tested in WB applications. This antibody reacts with Human, Mouse.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Lyophilized
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human LAMP1, different from the related mouse and rat sequences by one amino acid.
• UniProt ID: P11279
• MW: 90-120 kDa (Glycosylation)
• Tested applications: WB
• Application notes: Western blot, 0.1-0.5μg/ml, Human, Mouse. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Lysosome-associated membrane glycoprotein 1; LAMP-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of LAMP1 using anti-LAMP1 antibody (orb76335). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HeLa whole cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LAMP1 antigen affinity purified polyclonal antibody (Catalog # orb76335) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for LAMP1 at approximately 90-120 kDa (Glycosylation). The expected band size for LAMP1 is at 42 kDa.