L-Arginine decarboxylase/AZIN2 Antibody

• Catalog Number: orb381020
• Category: Antibodies
• Description: L-Arginine decarboxylase/AZIN2 Antibody
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Lyophilized
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Purification: Immunogen affinity purified.
• Immunogen: E. coli-derived human AZIN2 recombinant protein (Position: C111-Q301). Human AZIN2 shares 89.4% amino acid (aa) sequence identity with mouse AZIN2.
• UniProt ID: Q96A70
• MW: 39 kDa
• Tested applications: IHC, WB
• Application notes: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
• Cross Reactivity: No cross-reactivity with other proteins
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Antizyme inhibitor 2; AzI2; Arginine decarboxylase
• Note: For research use only

AZIN2 was detected in paraffin-embedded sections of mouse brain tissues using rabbit anti-AZIN2 Antigen Affinity purified polyclonal antibody at 1 µg/mL. The immunohistochemical section was developed using SABC method.

AZIN2 was detected in paraffin-embedded sections of rat brain tissues using rabbit anti-AZIN2 Antigen Affinity purified polyclonal antibody at 1 µg/mL. The immunohistochemical section was developed using SABC method.

IHC analysis of AZIN2 using anti-AZIN2 antibody. AZIN2 was detected in paraffin-embedded section of human testicar cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-AZIN2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Western blot analysis of AZIN2 expression in rat brain extract (lane 1), mouse brain extract (lane 2) and HELA whole cell lysates (lane 3). AZIN2 at 50KD was detected using rabbit anti-AZIN2 Antigen Affinity purified polyclonal antibody at 0.5 µg/mL. The blot was developed using chemiluminescence (ECL) method.