Anti-Ki67/MKI67 Antibody

• Catalog Number: orb180724
• Category: Antibodies
• Description: Anti-Ki67/MKI67 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Ki67 recombinant protein (Position: K2860-I3256).
• Antibody Type: Primary Antibody
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 358 kDa
• UniProt ID: P46013
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Antigen KI-67; MKI67
• Note: For research use only
• Application notes: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human Immunocytochemistry , 0.5-1μg/ml, Human, - Immunofluorescence, 5μg/ml, Human. Add 0.2ml of distilled water will yield a concentration of 500μg/ml
• Expiration Date: 12 months from date of receipt.

ICC analysis of Ki67 using anti-Ki67 antibody.Ki67 was detected in immunocytochemical section of HeLa cells.Biotinylated goat anti-rabbit IgG was used as secondary antibody.

WB analysis of Ki67 using anti-Ki67 antibody.Lane 1:HELA Cell;2:MCF-7 Cell; 3:COLO320 Cell;4:HEPG2 Cell;5:SKOV Cell.

IF analysis of Ki67 using anti-Ki67 antibody.Ki67 was detected in paraffin-embedded section of human colon organoid tissue.

IHC analysis of Ki67 using anti-Ki67 antibody. Ki67 was detected in paraffin-embedded section of human tonsil tissues.