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Anti-Ki67 Antibody-FITC labled
Catalog Number: orb1293852
| Catalog Number | orb1293852 |
|---|---|
| Category | Antibodies |
| Description | Rat monoclonal antibody against Ki67 conjugated to FITC |
| Target | MKI67 |
| Clonality | Monoclonal |
| Species/Host | Rat |
| Conjugation | FITC |
| Reactivity | Human, Mouse |
| Form/Appearance | Rat IgG2b kappa. Liquid in PBS, pH 7.3, and 0.02% sodium azide. |
| Buffer/Preservatives | Rat IgG2b kappa. Liquid in PBS, pH 7.3, and 0.02% sodium azide. |
| Immunogen | E. coli expressed, N-terminal His-Thioredoxin-tagged, partial mKi-67 (1816-2163 aa) recombinant protein |
| UniProt ID | P46013 |
| Tested applications | FC, IF |
| Dilution range | 10 μl / assay |
| Application notes | 1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add 10 μl labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 ml RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured). |
| Antibody Type | Primary Antibody |
| Clone Number | 11F6 |
| Source | Rat |
| Storage | Shipped and store at 4°C for one year. Do not freeze. |
| Alternative names | Antigen KI-67 |
| Note | For research use only |
| Entrez | 4288 |
