KCNJ16 Rabbit Polyclonal Antibody

SKU: orb670311

Description

Anti-KCNJ16 Antibody. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Monkey.

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: Western blot, 0.25-0.5μg/ml, Human, Monkey Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human
• Reactivity: Human, Monkey

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human KCNJ16 recombinant protein (Position: M1-M418).
• Target: Inward rectifier potassium channel 16
• Molecular Weight: 48 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
• Concentration: 500 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BIR9; KCNJ16; KIR5.1

Flow Cytometry analysis of U87 cells using anti-KCNJ16 antibody. Overlay histogram showing U87 cells (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-KCNJ16 Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Western blot analysis of KCNJ16 using anti-KCNJ16 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: monkey kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNJ16 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KCNJ16 at approximately 48 KD. The expected band size for KCNJ16 is at 48 KD.

Quick Database Links

Gene Symbol

Inward rectifier potassium channel 16

UniProt

Q9NPI9