KCNJ1 Rabbit Polyclonal Antibody

SKU: orb670310

Description

Anti-KCNJ1 Antibody. Tested in WB applications. This antibody reacts with Monkey, Mouse, Rat.

Research Area

Cell Biology, Signal Transduction

Images & Validation

• Tested Applications: WB
• Dilution Range: Western blot, 0.25-0.5μg/ml, Monkey, Mouse, Rat
• Reactivity: Monkey, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human KCNJ1, which shares 80% amino acid (aa) sequence identity with both mouse and rat KCNJ1.
• Target: ATP-sensitive inward rectifier potassium channel 1
• Molecular Weight: 45 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
• Concentration: 500 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

KCNJ1; KIR1.1; ROMK; ROMK1

Western blot analysis of KCNJ1 using anti-KCNJ1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: mouse kidney tissue lysates, Lane 4: monkey kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNJ1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KCNJ1 at approximately 45 KD. The expected band size for KCNJ1 is at 45 KD.

Quick Database Links

Gene Symbol

ATP-sensitive inward rectifier potassium channel 1

UniProt

P48048