Cart summary

You have no items in your shopping cart.

Anti-JNK1/2/3 (Phospho-T183/Y185) Antibody

Catalog Number: orb315601

DispatchUsually dispatched within 5-10 working days
$ 160.00
Catalog Numberorb315601
CategoryAntibodies
DescriptionRabbit polyclonal antibody to JNK1/2/3 (Phospho-T183/Y185)
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, WB
ReactivityHuman, Mouse, Rat
ImmunogenKLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T183/Y185 of human JNK1/2/3 protein. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1-500:1000
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetMAPK8; MAPK9; MAPK10
Entrez116554, 5602, 5599, 25272, 5601, 50658, 26419, 26420
UniProt IDP49186, P49187, Q91Y86, P45984, P49185, Q9WTU6, Q61831, P45983, P53779
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti MAPK8 antibody, anti JNK1 antibody, anti PRKM
Read more...
NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-JNK1/2/3 (Phospho-T183/Y185) Antibody

Western blot analysis of JNK1/2/3 (Phospho-T183/Y185) expression in BV2 (A), MCF7 (B), HCT116 (C), U87MG (D) whole cell lysates. (Predicted band size: 48; 52 kD; Observed band size: 46; 54 kD)

Anti-JNK1/2/3 (Phospho-T183/Y185) Antibody

Immunofluorescent analysis of JNK1/2/3 (Phospho-T183/Y185) staining in LS8 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

  • Anti-JNK1/2/3 (Phospho-T183/Y185) Antibody [orb1474463]

    IP,  WB

    Human, Rat

    Rabbit

    Monoclonal

    Unconjugated

    200 μl, 100 μl, 30 μl
  • JNK1/2/3 (Phospho-T183/Y185) peptide [orb315867]

    Blocking

    > 85%

    Synthetic

    5 mg, 1 mg