You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb315601 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to JNK1/2/3 (Phospho-T183/Y185) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Rat |
Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T183/Y185 of human JNK1/2/3 protein. The exact sequence is proprietary. |
Antibody Type | Primary Antibody |
Dilution range | WB: 1-500:1000 |
Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Conjugation | Unconjugated |
Target | MAPK8; MAPK9; MAPK10 |
Entrez | 116554, 5602, 5599, 25272, 5601, 50658, 26419, 26420 |
UniProt ID | P49186, P49187, Q91Y86, P45984, P49185, Q9WTU6, Q61831, P45983, P53779 |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative names | anti MAPK8 antibody, anti JNK1 antibody, anti PRKM Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of JNK1/2/3 (Phospho-T183/Y185) expression in BV2 (A), MCF7 (B), HCT116 (C), U87MG (D) whole cell lysates. (Predicted band size: 48; 52 kD; Observed band size: 46; 54 kD)
Immunofluorescent analysis of JNK1/2/3 (Phospho-T183/Y185) staining in LS8 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
IP, WB | |
Human, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |