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Anti-JNK1/2/3 (Phospho-T183) Antibody

Catalog Number: orb214454

DispatchUsually dispatched within 5-10 working days
$ 160.00
Catalog Numberorb214454
CategoryAntibodies
DescriptionRabbit polyclonal antibody to MAPK8
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Porcine, Rat
ImmunogenKLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T183 of human JNK1/2/3 protein. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetMAPK8; MAPK9; MAPK10
Entrez116554, 5602, 5599, 25272, 5601, 50658, 26419, 26420
UniProt IDP49186, P49187, Q91Y86, P45984, P49185, Q9WTU6, Q61831, P45983, P53779
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti MAPK8 antibody, anti JNK1 antibody, anti PRKM
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-JNK1/2/3 (Phospho-T183) Antibody

Western blot analysis of JNK1/2/3 (Phospho-T183) expression in HCT116 (A), MCF7 (B), A549 (C), rat brain (D), C6 (E) whole cell lysates. (Predicted band size: 48; 48; 52 kD; Observed band size: 46; 54 kD)

Anti-JNK1/2/3 (Phospho-T183) Antibody

Immunohistochemical analysis of JNK1/2/3 (Phospho-T183) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-JNK1/2/3 (Phospho-T183) Antibody

Immunofluorescent analysis of JNK1/2/3 (Phospho-T183) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Anti-JNK1/2/3 (Phospho-T183) Antibody

Direct ELISA antibody dose-response curve using Anti-JNK1/2/3 (Phospho-T183) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.

  • Anti-JNK1/2/3 (Phospho-T183/Y185) Antibody [orb315601]

    IF,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    200 μl, 100 μl, 30 μl
  • Anti-JNK1/2/3 (Phospho-T183/Y185) Antibody [orb1474463]

    IP,  WB

    Human, Rat

    Rabbit

    Monoclonal

    Unconjugated

    200 μl, 100 μl, 30 μl
  • JNK1/2/3 (Phospho-T183/Y185) peptide [orb315867]

    Blocking

    > 85%

    Synthetic

    5 mg, 1 mg