Anti-JAK2 Antibody

• Catalog Number: orb318917
• Category: Antibodies
• Description: Rabbit polyclonal antibody to JAK2 also known as JAK-2 whose expression can be detected in whole-body tissues ubiquitously. It is found in the extrinsic component of the nucleus. It can perform various functions like MAPK cascade mechanism and histone H3-Y41 phosphorylation. In the nucleus it plays a key role in chromatin by specifically mediating phosphorylation of 'Tyr-41' of histone H3 (H3Y41ph), a specific tag that promotes the exclusion of CBX5 (HP1 alpha) from chromatin. In the cytoplasm, it plays a pivotal role in signal transduction via its association with type I receptors such as growth hormone (GHR), prolactin (PRLR), leptin (LEPR), erythropoietin (EPOR), thrombopoietin (THPO); or type II receptors including IFN-alpha, IFN-beta, IFN-gamma and multiple interleukins.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IH, WB
• Reactivity: Human, Mouse, Porcine, Primate, Rabbit, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human JAK2. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1000, IHC-P: 1:100-200, IF/ICC: 1:100-500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: JAK2
• Entrez: 24514, 3717, 16452
• UniProt ID: Q62120, Q62689, O60674
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti-Tyrosine-protein kinase JAK2 antibody, anti-J
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Aguilera-Rojas, Matias et al. Deregulation of miR-27a may contribute to canine fibroblast activation after coculture with a mast cell tumour cell line FEBS Open Bio, 10, 802-816 (2020)

PubMed: 32133790

Western blot analysis of JAK2 expression in Jurkat (A), K562 (B), HEK293T (C) whole cell lysates. (Predicted band size: 130 kD; Observed band size: 125 kD)

Immunohistochemical analysis of JAK2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of JAK2 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.