Anti-Interleukin-6 IL6 Antibody

• Catalog Number: orb76360
• Category: Antibodies
• Description: Anti-Interleukin-6 IL6 Antibody. Tested in WB applications. This antibody reacts with Human.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Lyophilized
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Purification: Immunogen affinity purified.
• Immunogen: E. coli-derived human IL-6 recombinant protein (Position: P29-M212).
• UniProt ID: P05231
• MW: 20 kDa
• Tested applications: WB
• Application notes: Western blot, 0.1-0.5μg/ml, Human. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
• Cross Reactivity: No cross-reactivity with other proteins
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Interleukin-6; IL-6; B-cell stimulatory factor 2;
• Note: For research use only

Figure. Western blot analysis of IL-6 using anti-IL-6 antibody (orb76360). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane: Recombinant Human IL-6 Protein 0.5ng After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-6 antigen affinity purified polyclonal antibody (Catalog # orb76360) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for IL-6 at approximately 20KD. The expected band size for IL-6 is at 20KD.