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Interleukin-3 IL3 Rabbit Polyclonal Antibody
Description
Research Area
Images & Validation
−| Tested Applications | ELISA, WB |
|---|---|
| Dilution Range | ELISA, 0.1-0.5μg/ml, - Western blot, 0.1-0.5μg/ml, Rat |
| Reactivity | Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | E. coli-derived rat IL-3 recombinant protein (Position: S28-C166). Rat IL-3 shares 32% and 55% amino acid (aa) sequence identity with human and mouse IL-3, respectively. |
| Target | Interleukin-3 |
| Molecular Weight | 37 kDa |
| Purification | Immunogen affinity purified. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required. |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Anti-IL-3 antibody, Western blotting. All lanes: Anti IL-3 at 0.5 ug/ml. Lane 1: PC-12 Whole Cell Lysate at 40 ug. Lane 2: NRK Whole Cell Lysate at 40 ug. Lane 3: RH35 Whole Cell Lysate at 40 ug. Predicted bind size: 17 KD. Observed bind size: 37 KD.

Anti-IL-3 antibody, Western blotting. All lanes: Anti IL-3 at 0.5 ug/ml. WB: Recombinant Rat IL-3 Protein 0.5 ng. Predicted bind size: 15 KD. Observed bind size: 15 KD.
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Protocol Information
Interleukin-3 IL3 Rabbit Polyclonal Antibody (orb234273)
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