Anti-iNOS Antibody

• Catalog Number: orb214324
• Category: Antibodies
• Description: Rabbit polyclonal antibody to iNOS
• Target: NOS2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human iNOS. The exact sequence is proprietary.
• UniProt ID: P35228, P29477, Q06518
• Tested applications: IF, IH, WB
• Dilution range: WB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti HEP NOS antibody, anti HEP-NOS antibody, anti
• Note: For research use only
• Entrez: 18126, 4843, 24599

References

Xuejing Hou 1, Shanshan Xiao 2, Xiaohong Xu 3, Mingze Qin 4, Xuebing Cheng 4, Xiangping Xu Glycoprotein Non-metastatic Melanoma Protein B (GPNMB) Protects Against Neuroinflammation and Neuronal Loss in Pilocarpine-induced Epilepsy via the Regulation of Microglial Polarization Neuroscience, (2024)

PubMed: 38782114

Applications

WB

Reactivity

Rat

Western blot analysis of iNOS expression in MCF7 (A), A549 (B), Raw264.7 (C), PC12 (D), rat liver (E) whole cell lysates. (Predicted band size: 126; 131 kD; Observed band size: 130 kD)

Immunohistochemical analysis of iNOS staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of iNOS staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).