IL2 Receptor beta/IL2RB Antibody

SKU: orb334493

Description

Anti-IL2 Receptor beta/IL2RB Antibody. Tested in WB applications. This antibody reacts with Mouse.

Images & Validation

• Tested Applications: WB
• Reactivity: Mouse
• Application Notes:
  Western blot, 0.1-0.5μg/ml, Mouse. Add 0.2ml of distilled water will yield a concentration of 500ug/ml

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse IL2RB, different from the related human sequence by sixteen amino acids, and from the related rat sequence by fourteen amino acids.
• Molecular Weight: 75 kDa
• Purification: Immunogen affinity purified.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Disclaimer: For research use only

Alternative Names

Interleukin-2 receptor subunit beta; IL-2 receptor subunit beta; IL-2R subunit beta; IL-2RB; High affinity IL-2 receptor subunit beta; p70-75; CD122; Il2rb

Western blot analysis of IL2RB using anti-IL2RB antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: Mouse Lung Tissue Lysate, Lane 2: Mouse Spleen Tissue Lysate, Lane 3: Mouse Testis Tissue Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL2RB antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL2RB at approximately 75 kDa. The expected band size for IL2RB is at 75 kDa.

Quick Database Links

UniProt

P16297