IL17/IL17A Antibody

• Catalog Number: orb76367
• Category: Antibodies
• Description: Anti-IL17/IL17A Antibody. Tested in ELISA, WB applications. This antibody reacts with Mouse.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Mouse
• Form/Appearance: Lyophilized
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Purification: Immunogen affinity purified.
• Immunogen: E. coli-derived mouse IL-17 recombinant protein (Position: A26-A158).
• UniProt ID: Q62386
• MW: 30 kDa
• Tested applications: ELISA, WB
• Application notes: ELISA, 0.1-0.5μg/ml, -Western blot, 0.1-0.5μg/ml, Mouse. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
• Cross Reactivity: No cross-reactivity with other proteins
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Interleukin-17A; IL-17; IL-17A; Cytotoxic T-lympho
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Figure. Western blot analysis of IL-17 using anti-IL-17 antibody (orb76367). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane: Recombinant Mouse IL-17 Protein 0.5ng After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-17 antigen affinity purified polyclonal antibody (Catalog # orb76367) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for IL-17 at approximately 30KD. The expected band size for IL-17 is at 30KD.