Anti-IL16 Antibody

• Catalog Number: orb221297
• Category: Antibodies
• Description: Anti-IL16 Antibody
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Lyophilized
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Immunogen: E.coli-derived human IL-16 recombinant protein (Position: S1212-S1332). Human IL-16 shares 86% amino acid (aa) sequence identity with mouse IL-16.
• UniProt ID: Q14005
• MW: 45-55 kDa
• Tested applications: ELISA, ICC, IHC, WB
• Application notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human Immunocytochemistry , 0.5-1μg/ml, Human, -Western blot, 0.1-0.5μg/ml, Human ELISA, 0.1-0.5μg/ml, -. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Pro-interleukin-16; Interleukin-16; IL-16; Lymphoc
• Note: For research use only

Anti-IL-16 antibody, IHC(P): Human Tonsil Tissue.

Anti-IL-16 antibody, Western blotting. All lanes: Anti IL-16 at 0.5 ug/ml. WB: Recombinant Human IL-16 Protein 0.5 ng. Predicted bind size: 17 KD. Observed bind size: 17 KD.

IHC analysis of IL16 using anti-IL16 antibody. IL16 was detected in immunocytochemical section of human cord blood. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 µg/ml rabbit anti-IL16 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Western blot analysis of IL16 using anti-IL16 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: human U937 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL16 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL16 at approximately 45-55 KD. The expected band size for IL16 is at 142 KD.