IL10 Antibody

SKU: orb692186

Description

Anti-IL10 Antibody. Tested in ELISA, IHC applications. This antibody reacts with Mouse, Rat.

Images & Validation

• Tested Applications: ELISA, IHC
• Reactivity: Mouse, Rat
• Application Notes:
  Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat ELISA, 0.1-0.5μg/ml,. Add 0.2ml of distilled water will yield a concentration of 500μg/ml

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived rat IL10 recombinant protein (Position: K52-A170).
• Target: Receptor tyrosine-protein kinase erbB-2
• Molecular Weight: 19 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Disclaimer: For research use only

Alternative Names

Interleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIF; IL10

IHC analysis of IL10 using anti-IL10 antibody. IL10 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-IL10 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of IL10 using anti-IL10 antibody. IL10 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-IL10 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Quick Database Links

Gene Symbol

Receptor tyrosine-protein kinase erbB-2

UniProt

P29456