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Anti-IL10 Antibody

Catalog Number: orb692186

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb692186
CategoryAntibodies
DescriptionAnti-IL10 Antibody. Tested in ELISA, IHC applications. This antibody reacts with Mouse, Rat.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, IHC
ReactivityMouse, Rat
IsotypeRabbit IgG
ImmunogenE.coli-derived rat IL10 recombinant protein (Position: K52-A170).
Antibody TypePrimary Antibody
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form/AppearanceLyophilized
ConjugationUnconjugated
MW19 kDa
UniProt IDP29456
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesInterleukin-10; IL-10; Cytokine synthesis inhibito
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NoteFor research use only
Application notesImmunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat ELISA, 0.1-0.5μg/ml,. Add 0.2ml of distilled water will yield a concentration of 500μg/ml
Expiration Date12 months from date of receipt.
Anti-IL10 Antibody

IHC analysis of IL10 using anti-IL10 antibody. IL10 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-IL10 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-IL10 Antibody

IHC analysis of IL10 using anti-IL10 antibody. IL10 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-IL10 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

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    ELISA,  IHC,  WB

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