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Anti-IL10 Antibody
Catalog Number: orb412952
| Catalog Number | orb412952 |
|---|---|
| Category | Antibodies |
| Description | Anti-IL10 Antibody. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Mouse, Rat |
| Form/Appearance | Lyophilized |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Immunogen | E. coli-derived mouse IL10 recombinant protein (Position: S19-S178). |
| UniProt ID | P18893 |
| MW | 19 kDa |
| Tested applications | ELISA, WB |
| Application notes | Western blot,0.1-0.5μg/ml ELISA (Cap),1-5μg/ml. Add 0.2ml of distilled water will yield a concentration of 500ug/ml |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Interleukin-10; IL-10; Cytokine synthesis inhibito Read more... |
| Note | For research use only |
Sandwich ELISA - Recombinant mouse IL10 protein standard curve. Use in combination with reagents from Mouse IL10 ELISA Kit EZ-Set (DIY Antibody Pairs).
Western blot analysis of IL10 using anti-IL10 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: mouse brain tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL10 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL10 at approximately 19KD. The expected band size for IL10 is at 21KD.
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