Anti-IL-31Ra Reference Antibody (nemolizumab)

• Catalog Number: orb1818360
• Category: Antibodies
• Description: Recombinant Antibody
• Clonality: Monoclonal
• Tested applications: ELISA, FC
• Reactivity: Human, Monkey
• Isotype: IgG2SA
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Form/Appearance: Purified monoclonal antibody supplied in PBS, pH 6.0, without preservative.This antibody is purified through a protein A column.
• Conjugation: Unconjugated
• MW: 144.92
• UniProt ID: Q8NI17
• Protein Sequence: IL-31Ra
• Expression System: CHO Cell
• Endotoxins: < 0.001EU/μg,determined by LAL method.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Interleukin-31 receptor subunit alpha, IL-31 recep
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

The purity of Anti-IL-31Ra Reference Antibody (nemolizumab) is more than 100%, determined by SEC-HPLC.

Immobilized human IL31R His at 2 µg/mL can bind Anti-IL-31Ra Reference Antibody (nemolizumab), EC50=0.0153 µg/mL

Anti-IL-31Ra Reference Antibody (nemolizumab) P-STAT3 Assay was evaluated using Du145. The IC50 was approximately 0.1068 ug/ml.

Anti-IL-31Ra Reference Antibody (nemolizumab) Activation inhibition was evaluated using Hacat. The max induction fold was approximately 1.86.

Anti-IL-31Ra Reference Antibody (nemolizumab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%

Human IL31R HEK293 cells were stained with Anti-IL-31Ra Reference Antibody (nemolizumab) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC181=0.307 µg/mL