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Catalog Number | orb1806167 |
---|---|
Category | Antibodies |
Description | Anti-IL-31Ra Reference Antibody is expressed from CHO. The heavy chain type is huIgG2, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa. |
Clonality | Monoclonal |
Tested applications | ELISA, FA, FACS, Kinetics |
Reactivity | Human |
Isotype | IgG |
Purity | > 95% |
Conjugation | Unconjugated |
MW | 145.5 kDa |
Target | IL-31Ra |
Endotoxins | < 1 EU/mg |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | 100 mM Pro-Ac 20 mM Arg pH 5.0 |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Anti-IL-31Ra Reference Antibody (nemolizumab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Anti-IL-31Ra Reference Antibody (nemolizumab)is more than 100% ;determined by SEC-HPLC.
Immobilized human IL31R His at 2 μg/mL can bind Anti-IL-31Ra Reference Antibody (nemolizumab);EC50=0.0153μg/mL.
Human IL31R HEK293 cells were stained with Anti-IL-31Ra Reference Antibody (nemolizumab) and negative control protein respectively; washed and then followed by PE and analyzed with FACS; EC181=0.307 μg/mL.
Anti-IL-31Ra Reference Antibody (nemolizumab) P-STAT3 Assay was evaluated using Du145. The IC50 was approximately 0.1068 ug/ml.
Anti-IL-31Ra Reference Antibody (nemolizumab) Activation inhibition was evaluated using Hacat. The max induction fold was approximately 1.86.
ELISA, FC | |
Human, Monkey | |
Monoclonal | |
Unconjugated |