Anti-IL-31Ra Reference Antibody (nemolizumab)

• Catalog Number: orb1806167
• Category: Antibodies
• Description: Anti-IL-31Ra Reference Antibody is expressed from CHO. The heavy chain type is huIgG2, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.
• Target: IL-31Ra
• Clonality: Monoclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 100 mM Proline/Acetic acid 20 mM Arginine pH 5.0
• Purity: > 95%
• MW: 145.5 kDa
• Tested applications: ELISA, FA, FACS, Kinetics
• Endotoxins: < 1 EU/mg
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only

Anti-IL-31Ra Reference Antibody (nemolizumab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Anti-IL-31Ra Reference Antibody (nemolizumab)is more than 100% ;determined by SEC-HPLC.

Immobilized human IL31R His at 2 μg/mL can bind Anti-IL-31Ra Reference Antibody (nemolizumab);EC50=0.0153μg/mL.

Human IL31R HEK293 cells were stained with Anti-IL-31Ra Reference Antibody (nemolizumab) and negative control protein respectively; washed and then followed by PE and analyzed with FACS; EC181=0.307 μg/mL.

Anti-IL-31Ra Reference Antibody (nemolizumab) P-STAT3 Assay was evaluated using Du145. The IC50 was approximately 0.1068 ug/ml.

Anti-IL-31Ra Reference Antibody (nemolizumab) Activation inhibition was evaluated using Hacat. The max induction fold was approximately 1.86.