IL-1RA Rabbit Polyclonal Antibody

SKU: orb378130

Description

The IL-1RA Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human IL-1RA .Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1:500-2000, IF/ICC: 1:50-200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein of human IL-1RA
• Target: IL1RN
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IL1F3; IL1RA; Interleukin-1 receptor antagonist protein; IL-1RN; IL-1ra; IRAP; ICIL-1RA; IL1 inhibitor; Anakinra

Western blot analysis of IL-1RA expression in Hela (A), NIH3T3 (B), HL60 (C) whole cell lysates. (Predicted band size: 16; 17; 19; 20 kD; Observed band size: 18 kD)

Immunofluorescent analysis of IL-1RA staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

Gene Symbol

IL1RN

UniProt

P25085, P25086, P18510

NCBI Gene ID

3557, 60582, 16181