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Anti-IL-17A/Il17a Antibody
Catalog Number: orb865532
| Catalog Number | orb865532 |
|---|---|
| Category | Antibodies |
| Description | Anti-IL-17A/Il17a Antibody. Tested in ELISA, IHC applications. This antibody reacts with Mouse, Rat. |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ELISA, IHC |
| Reactivity | Mouse, Rat |
| Isotype | Rabbit IgG |
| Immunogen | E.coli-derived rat IL-17A/Il17a recombinant protein (Position: A18-S150). |
| Antibody Type | Primary Antibody |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Form/Appearance | Lyophilized |
| Conjugation | Unconjugated |
| MW | 27 kDa |
| UniProt ID | Q61453 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Interleukin-17A; IL-17; IL-17A; Cytotoxic T-lympho Read more... |
| Note | For research use only |
| Application notes | Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat ELISA, 0.1-0.5 μg/ml, Rat. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml |
| Expiration Date | 12 months from date of receipt. |
IHC analysis of IL-17A/Il17a using anti-IL-17A/Il17a antibody. IL-17A/Il17a was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-IL-17A/Il17a Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of IL-17A/Il17a using anti-IL-17A/Il17a antibody. IL-17A/Il17a was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-IL-17A/Il17a Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
