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Anti-IL-10/IL10 Antibody
Catalog Number: orb1184717
| Catalog Number | orb1184717 |
|---|---|
| Category | Antibodies |
| Description | Anti-IL-10/IL10 Antibody. Tested in ELISA, IHC applications. This antibody reacts with Human. |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Lyophilized |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Immunogen | E.coli-derived human IL-10/IL10 recombinant protein (Position: K137-N178). |
| UniProt ID | P22301 |
| MW | 19 kDa |
| Tested applications | ELISA, IHC |
| Application notes | Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml/ml, Human ELISA, 0.1-0.5 μg/ml/ml, Human. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Interleukin-10; IL-10; Cytokine synthesis inhibito Read more... |
| Note | For research use only |
IHC analysis of IL-10/IL10 using anti-IL-10/IL10 antibody. IL-10/IL10 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-IL-10/IL10 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
