Anti-IGFBP3 Antibody

• Catalog Number: orb402359
• Category: Antibodies
• Description: Anti-IGFBP3 Antibody. Tested in ELISA, WB applications. This antibody reacts with Human.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human IGFBP3 recombinant protein (Position: A29-K267).
• Antibody Type: Primary Antibody
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 37 kDa
• UniProt ID: P17936
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Insulin-like growth factor-binding protein 3; IBP-
• Note: For research use only
• Application notes: Western blot, 0.1-0.5μg/ml, human ELISA (Cap), 1-5μg/ml, human. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
• Expiration Date: 12 months from date of receipt.

Sandwich ELISA - Recombinant human IGFBP3 protein standard curve. Use in combination with reagents from Human IGFBP3 ELISA Kit EZ-Set (DIY Antibody Pairs).

Western blot analysis of IGFBP3 using anti-IGFBP3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human IGFBP3 protein 1 ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP3 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IGFBP3 at approximately 37KD. The expected band size for IGFBP3 is at 29KD.