IGF1 Rabbit Polyclonal Antibody

SKU: orb669229

Description

Anti-IGF1 Antibody. Tested in ELISA, Flow Cytometry, IHC applications. This antibody reacts with Human.

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Epigenetics & Chromatin, Metabolism Research, Molecular Biology, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: ELISA, FC, IHC
• Dilution Range: Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human ELISA (Cap), 1-5μg/ml, Human
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human IGF1 recombinant protein (Position: P50-A118).
• Target: Insulin-like growth factor 1
• Molecular Weight: 25 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
• Concentration: 500 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Angiogenin; 3.1.27.-; Ribonuclease 5; RNase 5; ANG; RNASE5

Flow Cytometry analysis of Hela cells using anti-IGF1 antibody. Overlay histogram showing Hela cells (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-IGF1 Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

IHC analysis of IGF1 using anti-IGF1 antibody. IGF1 was detected in paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-IGF1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Quick Database Links

Gene Symbol

Insulin-like growth factor 1

UniProt

P05019