Anti-IFN gamma Receptor beta/AF-1/IFNGR2 Antibody

• Catalog Number: orb654333
• Category: Antibodies
• Description: Anti-IFN gamma Receptor beta/AF-1/IFNGR2 Antibody. Tested in ELISA, WB applications. This antibody reacts with Mouse.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Mouse
• Form/Appearance: Lyophilized
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Purification: Immunogen affinity purified.
• Immunogen: E.coli-derived mouse IFN gamma Receptor beta/AF-1/IFNGR2 recombinant protein (Position: A20-P332).
• UniProt ID: Q63953
• MW: 55 kDa
• Tested applications: ELISA, WB
• Application notes: Western blot, 0.25-0.5μg/ml, Mouse ELISA, 0.1-0.5μg/ml, -. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
• Cross Reactivity: No cross-reactivity with other proteins.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IFNGR2 protein; Interferon gamma receptor 2; IFNGR
• Note: For research use only

Western blot analysis of IFN Gamma Receptor Beta/AF-1/Ifngr2 using anti-IFN Gamma Receptor Beta/AF-1/Ifngr2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates, Lane 2: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IFN Gamma Receptor Beta/AF-1/Ifngr2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IFN Gamma Receptor Beta/AF-1/Ifngr2 at approximately 55 KD. The expected band size for IFN Gamma Receptor Beta/AF-1/Ifngr2 is at 38 KD.