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Anti-HuC Antibody

Catalog Number: orb340994

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb340994
CategoryAntibodies
DescriptionRabbit polyclonal antibody to ELAVL3
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, IP, WB
ReactivityHuman, Mouse, Rat
ImmunogenKLH-conjugated synthetic peptide of human HuC
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500-1:1000, IHC-P: 1:50-1:200, IP: 1:20-1:50
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetELAVL3
Entrez1995, 15571
UniProt IDQ60900, Q14576
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesHUC; PLE21; ELAV-like protein 3; Hu-antigen C; HuC
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-HuC Antibody

Western blot analysis of HuC expression in mouse brain (A), rat brain (B) whole cell lysates. (Predicted band size: 38; 39 kD; Observed band size: 38 kD)

Anti-HuC Antibody

Immunohistochemical analysis of HuC staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-HuC Antibody

Immunofluorescent analysis of HuC staining in SHSY5Y cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

  • ELAVL3 Antibody [orb752480]

    ELISA,  IHC

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 50 μl
  • ELAVL3 Antibody, Biotin conjugated [orb752481]

    ELISA

    Human

    Rabbit

    Polyclonal

    Biotin

    100 μl, 50 μl
  • ELAVL3 Antibody, FITC conjugated [orb752482]

    Human

    Rabbit

    Polyclonal

    FITC

    100 μl, 50 μl
  • ELAVL3 Antibody, HRP conjugated [orb752483]

    ELISA

    Human

    Rabbit

    Polyclonal

    HRP

    100 μl, 50 μl
  • ELAVL3 Antibody [orb678097]

    ELISA,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl