CD38 Antibody (PE)

• Catalog Number: orb43854
• Category: Antibodies
• Description: Mouse monoclonal antibody against CD38 conjugated to PE
• Target: CD38
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Conjugation: PE
• Reactivity: Human
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Immunogen: Human thymocytes in foetus
• UniProt ID: P28907
• RRID: AB_10991906
• Tested applications: FC
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
• Antibody Type: Primary Antibody
• Clone Number: HIT2
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Alternative names: ADPRC1, cADPr hydrolase 1, T10, NAD(+) nucleosidas
• Research Area: Epigenetics, Stem Cells
• Note: For research use only
• Entrez: 952
• Expiration Date: 12 months from date of receipt.

Separation of human monocytes (red-filled) from CD38 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD38 (HIT2) PE antibody (20 µl reagent / 100 µl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD38 (HIT2) PE antibody (20 µl reagent / 100 µl of peripheral whole blood).

Expression profiling on peripheral blood subsets using Anti-human CD38 PE antibody (clone HIT2). Adaptive panel. HCDM CDMaps standardized procedures were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes. Suspension of blood leukocytes isolated from buffy coats (2 x 10^6 cells) was added to the mixture of anti-human CD38 PE antibody (clone HIT2, 2 µg/ml in stained blood sample) and Monocyte Blocking Buffer, vortexed and incubated for 20 min. Next, optimized backbone antibody panel (HLDA Adaptive) was added to test tubes, vortexed and incubated for 20 min. The residual erythrocytes were lysed with 2 ml of 10× diluted EXCELLYSE Easy solution and incubated for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.

Expression profiling on peripheral blood subsets using Anti-human CD38 PE antibody (clone HIT2). Innate panel. HCDM CDMaps standardized procedures were used for cell isolation and surface staining of blood leukocytes, with the modification of staining protocol using cytometry test tubes. Suspension of blood leukocytes isolated from buffy coats (2 x 10^6 cells) was added to the mixture of anti-human CD38 PE antibody (clone HIT2, 2 µg/ml in stained blood sample) and Monocyte Blocking Buffer, vortexed and incubated for 20 min. Next, optimized backbone antibody panel (HLDA Innate) was added to test tubes, vortexed and incubated for 20 min. The residual erythrocytes were lysed with 2 ml of 10× diluted EXCELLYSE Easy solution and incubated for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition.