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Anti-Hu CD38 FITC
Catalog Number: orb2281308
| Catalog Number | orb2281308 |
|---|---|
| Category | Antibodies |
| Description | The mouse monoclonal antibody HB7 (HB-7) recognizes an extracellular epitope within amino acids 273-285 of human CD38, a 45 kDa type II transmembrane glycoprotein strongly expressed mainly on plasma cells and activated T and B lymphocytes; it is an antigenic marker of lymphoid cells. Its binding is blocked by daratumumab |
| Target | CD38 |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 kappa |
| Conjugation | FITC |
| Reactivity | Human |
| Form/Appearance | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Purity | Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography |
| Immunogen | BJAB cell line |
| UniProt ID | P28907 |
| Tested applications | FC, ICC, WB |
| Dilution range | Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests |
| Application notes | Unconjugated version: orb1952612 |
| Clone Number | HB7 |
| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze |
| Alternative names | ADPRC1, cADPr hydrolase 1, T10, NAD(+) nucleosidas Read more... |
| Background | CD38 (NAD+ glycohydrolase) is a type II transmembr Read more... |
| Note | For research use only |
| Entrez | 952 |
Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats. Mouse monoclonal anti-human CD38 FITC antibody (clone HB7) was used in concentration 0.5 µg/ml in stained blood sample (2 x 106 cells)
Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats. Mouse monoclonal anti-human CD38 FITC antibody (clone HB7) in concentration 0.5 µg/ml in stained stained blood sample (2 x 106 cells) and Mouse monoclonal anti-human CD27 Pacific Blue™ antibody (clone LT27) was used in concentration 2 µg/ml, respectively
Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats. Mouse monoclonal anti-human CD38 FITC antibody (clone HB7) in concentration 0.5 µg/ml in stained stained blood sample (2 x 106 cells) and Mouse monoclonal anti-human CD138 APC antibody (clone MI15) in concentration 2 µg/ml, respectively
Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats. Suspension of blood leukocytes (2 x 106 cells) was added to the mixture of CD38 FITC antibody (clone HB7, 0.5 µg/ml in stained blood sample), backbone antibody conjugates and Monocyte Blocking Buffer, vortexed and incubated for 20 min. Stained sample was fixed with 2 ml of 10× diluted EXCELLYSE Easy solution for 10 min. Finally, samples were centrifuged (670 g, 5 min.), supernatant removed and the cell pellet was resuspended in 200 µl of PBS for acquisition
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