CD105 Antibody (APC)

• Catalog Number: orb1909791
• Category: Antibodies
• Description: CD105 (endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbetaR-2 as a receptor for TGFbeta-1 and TGFbeta-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFbeta-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.
• Target: CD105
• Clonality: Monoclonal
• Isotype: Mouse IgG2a
• Conjugation: APC
• Reactivity: Human, Porcine
• Buffer/Preservatives: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
• Immunogen: Recombinant Vaccinia virus containing the human CD105 (L-isoform) cDNA.
• UniProt ID: P17813
• Tested applications: FC
• Application notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
• Clone Number: MEM-229
• Storage: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
• Alternative names: Endoglin, END, ENG, HHT1, ORW1
• Note: For research use only
• Entrez: 2022
• Expiration Date: 12 months from date of receipt.

Separation of HUVEC cells stained using anti-human CD105 (MEM-229) APC antibody (10 μl reagent per million cells in 100 μl of cell suspension, red-filled) from HUVEC cells stained using mouse IgG2a isotype control (MOPC-173) APC antibody (concentration in sample 5 μg/ml, same as CD105 APC concentration, black-dashed) in flow cytometry analysis (surface staining).