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Anti-HLA-G Purified Low Endotoxin
Catalog Number: orb154421
| Catalog Number | orb154421 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody to HLA-G which is a member of the MHC class I family that is observed in trophoblasts. It involved in the presentation of foreign antigens to the immune system also participate in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells. |
| Clonality | Monoclonal |
| Clone Number | 87G |
| Tested applications | ELISA, FC, IHC-Fr |
| Reactivity | Human |
| Isotype | Mouse IgG2a |
| Immunogen | HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin. |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/ml |
| Dilution range | Flow cytometry: Extracellular and intracellular staining; recommended dilution: 2 μg/ml; positive control: JEG-3 human choriocarcinoma epithelial cell line. Immunohistochemistry (frozen sections): Recommended dilution: 10 μg/ml, incubation: 20 min at 25°C; positive tissue: extravillous cytotrophoblast. ELISA: Recommended dilution: 1 μg/ml; positive control: JEG-3 human choriocarcinoma epithelial cell line. The antibody 87G has been tested as the capture antibody in a sandwich ELISA for analysis of human HLA-G in combination with antibody W6/32. Functional application: The antibody 87G blocks interaction of HLA-G with inhibitory receptors. |
| Purity | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
| Target | HLA-G |
| Entrez | 3135 |
| UniProt ID | P17693 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4 |
| Note | For research use only |
| Application notes | Low Endotoxin, Azide free format. Product does not contain preservatives and is intended primarily for functional applications. Preservative containing buffers are available for IHC applications |
| Expiration Date | 12 months from date of receipt. |
Separation of HLA-G transfected LCL cells (red-filled) from non-transfected LCL cells (black-dashed) in flow cytometry analysis (surface staining) stained using anti-HLA-G (87G) purified antibody (concentration in sample 10 μg/ml, GAM APC).
