Anti-Histone H3 (DiMethyl K27) Antibody

• Catalog Number: orb1421507
• Category: Antibodies
• Description: Mouse monoclonal antibody to Histone H3 (DiMethyl K27).
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• UniProt ID: P68431, Q71DI3, P84243
• Tested applications: IHC, IP, WB
• Dilution range: WB: WB (1/1000 - 1/3000), IH (1/200 - 1/500), IP (1/50 - 1/200), IP: WB (1/1000 - 1/3000), IH (1/200 - 1/500), IP (1/50 - 1/200)
• Clone Number: HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HI
• Note: For research use only

Western blot analysis of Histone H3 (DiMethyl K27) expression in Hela (A) whole cell lysates.

Immunoprecipitation of Histone H3 (DiMethyl K27) from 0.5mg Hela whole cell extract lysate, using Anti-Histone H3 (DiMethyl K27) Antibody.

Immunohistochemical analysis of Histone H3 (DiMethyl K27) staining in human skin formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.