Anti-HIST1H2BL Antibody

• Catalog Number: orb1880629
• Category: Antibodies
• Description: Rabbit polyclonal antibody to HIST1H2BL
• Clonality: Polyclonal
• Tested applications: FC, WB
• Reactivity: Human
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-terminal region of human HIST1H2BL. The exact sequence is proprietary.
• Dilution range: WB (1/500 - 1/1000), FC (1/10 - 1/50)
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Entrez: 8340
• UniProt ID: Q99880
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Alternative names: H2BFC; Histone H2B type 1-L; Histone H2B.c; H2B/c
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of HIST1H2BL expression in K562 (A) whole cell lysates. (Predicted band size: 13 kD; Observed band size: 13 kD)

Flow cytometric analysis of K562 cells using Anti-HIST1H2BL Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Rabbit IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.