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Anti-HIF-1 alpha/HIF1A Antibody

Catalog Number: orb570389

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb570389
CategoryAntibodies
DescriptionAnti-HIF-1 alpha/HIF1A Antibody. Tested in ELISA, WB applications. This antibody reacts with Human.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, WB
ReactivityHuman
IsotypeRabbit IgG
ImmunogenE.coli-derived human HIF-1 alpha/HIF1A recombinant protein (Position: H197-R718).
Antibody TypePrimary Antibody
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form/AppearanceLyophilized
ConjugationUnconjugated
MW123 kDa
UniProt IDQ16665
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesHypoxia-inducible factor 1-alpha; HIF-1-alpha; HIF
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NoteFor research use only
Application notesWestern blot, 0.25-0.5μg/ml, Human ELISA, 0.1-0.5μg/ml, -. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
Expiration Date12 months from date of receipt.
Anti-HIF-1 alpha/HIF1A Antibody

Western blot analysis of HIF1A using anti-HIF1A antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF1A antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HIF1A at approximately 123 KD. The expected band size for HIF1A is at 93 KD.

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