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Anti-HIC5/TGFB1I1 Antibody

Catalog Number: orb865471

DispatchCurrently estimated at 1-3 months
$ 210.00
Catalog Numberorb865471
CategoryAntibodies
DescriptionAnti-HIC5/TGFB1I1 Antibody. Tested in ELISA, WB applications. This antibody reacts with Human, Monkey.
ClonalityPolyclonal
Species/HostRabbit
IsotypeRabbit IgG
ConjugationUnconjugated
ReactivityHuman, Monkey
Form/AppearanceLyophilized
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
PurificationImmunogen affinity purified.
ImmunogenE.coli-derived human HIC5/TGFB1I1 recombinant protein (Position: E268-A447).
UniProt IDO43294
MW75 kDa
Tested applicationsELISA, WB
Application notesWestern blot, 0.25-0.5 μg/ml, Monkey ELISA, 0.1-0.5 μg/ml, -. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml
Cross ReactivityNo cross-reactivity with other proteins.
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesSolute carrier family 12 member 5; Electroneutral
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NoteFor research use only
Anti-HIC5/TGFB1I1 Antibody

Western blot analysis of HIC5/TGFB1I1 using anti-HIC5/TGFB1I1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIC5/TGFB1I1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HIC5/TGFB1I1 at approximately 75 kDa. The expected band size for HIC5/TGFB1I1 is at 75 kDa.

  • Anti-HIC5/TGFB1I1 Antibody [orb2599547]

    ELISA,  WB

    Human, Monkey

    Rabbit

    Polyclonal

    iFluor647

    100 μg
  • Anti-HIC5/TGFB1I1 Antibody [orb2599548]

    ELISA,  WB

    Human, Monkey

    Rabbit

    Polyclonal

    PE

    100 μg
  • Anti-HIC5/TGFB1I1 Antibody [orb2599549]

    ELISA,  WB

    Human, Monkey

    Rabbit

    Polyclonal

    APC

    100 μg
  • Anti-HIC5/TGFB1I1 Antibody [orb2599550]

    ELISA,  WB

    Human, Monkey

    Rabbit

    Polyclonal

    HRP

    100 μg
  • Anti-HIC5/TGFB1I1 Antibody [orb2599551]

    ELISA,  WB

    Human, Monkey

    Rabbit

    Polyclonal

    FITC

    100 μg