Heparanase 1/HPSE Rabbit Polyclonal Antibody

SKU: orb259604

Description

Anti-Heparanase 1/HPSE Antibody. Tested in WB applications. This antibody reacts with Human, Rat.

Research Area

Cancer Biology, Cell Biology, Signal Transduction

Images & Validation

• Tested Applications: WB
• Dilution Range: Western blot, 0.1-0.5μg/ml, Human, Rat
• Reactivity: Human, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Heparanase 1, different from the related mouse and rat sequences by eight amino acids.
• Target: Heparanase
• Molecular Weight: 61 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Heparanase; 3.2.1.166; Endo-glucoronidase; Heparanase-1; Hpa1; Heparanase 8 kDa subunit; Heparanase 50 kDa subunit; HPSE; HEP, HPA, HPA1, HPR1, HPSE1, HSE1

Western blot analysis of Heparanase 1 using anti-Heparanase 1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Liver Tissue Lysate at 50 ug, Lane 2: Human Placenta Tissue Lysate at 50 ug, Lane 3: A549 Whole Cell Lysate at 40 ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Heparanase 1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Heparanase 1 at approximately 61 kDa. The expected band size for Heparanase 1 is at 61 kDa.

Quick Database Links

Gene Symbol

Heparanase

UniProt

Q9Y251