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| Catalog Number | orb2622447 |
|---|---|
| Category | Antibodies |
| Description | Anti-HEF1/NEDD9 Antibody. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | FITC |
| Reactivity | Human, Monkey, Mouse, Rat |
| Form/Appearance | Lyophilized |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Purification | Immunogen affinity purified. |
| Immunogen | E.coli-derived human HEF1 recombinant protein (Position: K273-E421). Human HEF1 shares 83% amino acid (aa) sequence identity with mouse HEF1. |
| UniProt ID | Q14511 |
| MW | 93-105 kDa |
| Tested applications | FC, ICC, IF, IHC, WB |
| Application notes | Western blot, 0.1-0.5μg/ml, Human, Monkey, Mouse, Rat Immunohistochemistry, 2-5μg/ml, Human Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human. Add 0.2ml of distilled water will yield a concentration of 500ug/ml |
| Cross Reactivity | No cross-reactivity with other proteins |
| Antibody Type | Primary Antibody |
| Storage | Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. |
| Alternative names | Enhancer of filamentation 1; hEF1; CRK-associated Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
FC, ICC, IF, IHC, WB | |
Human, Monkey, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human, Monkey, Mouse, Rat | |
Rabbit | |
Polyclonal | |
iFluor647 |
FC, ICC, IF, IHC, WB | |
Human, Monkey, Mouse, Rat | |
Rabbit | |
Polyclonal | |
PE |
FC, ICC, IF, IHC, WB | |
Human, Monkey, Mouse, Rat | |
Rabbit | |
Polyclonal | |
APC |
FC, ICC, IF, IHC, WB | |
Human, Monkey, Mouse, Rat | |
Rabbit | |
Polyclonal | |
HRP |
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