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Anti-HE4/WFDC2 Antibody

Catalog Number: orb443134

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb443134
CategoryAntibodies
DescriptionAnti-HE4/WFDC2 Antibody. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, IHC, WB
ReactivityHuman, Mouse, Rat
IsotypeRabbit IgG
ImmunogenE. coli-derived human HE4 recombinant protein (Position: E31-F124).
Antibody TypePrimary Antibody
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form/AppearanceLyophilized
ConjugationUnconjugated
MW15 kDa
UniProt IDQ14508
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesWAP four-disulfide core domain protein 2; Epididym
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NoteFor research use only
Application notesWestern blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml ELISA, 0.1-0.5μg/ml. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
Expiration Date12 months from date of receipt.
Anti-HE4/WFDC2 Antibody

IHC analysis of HE4 using anti-HE4 antibody. HE4 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-HE4 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-HE4/WFDC2 Antibody

IHC analysis of HE4 using anti-HE4 antibody. HE4 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-HE4 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-HE4/WFDC2 Antibody

IHC analysis of HE4 using anti-HE4 antibody. HE4 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-HE4 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-HE4/WFDC2 Antibody

Western blot analysis of HE4 using anti-HE4 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MDA-MB-231 whole cell lysates, Lane 3: human MDA-MB-453 whole cell lysates, Lane 4: rat thymus tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HE4 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HE4 at approximately 15KD. The expected band size for HE4 is at 13KD.

  • Anti-HE4/Wfdc2 Antibody [orb402508]

    ELISA,  IHC,  WB

    Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    10 μg, 100 μg
  • Anti-HE4/WFDC2 Antibody [orb371697]

    ELISA,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    10 μg, 100 μg
  • Anti-HE4 WFDC2 Rabbit Monoclonal Antibody [orb548084]

    FC,  ICC,  IF,  IP,  WB

    Human, Mouse

    Rabbit

    Monoclonal

    Unconjugated

    30 μl, 100 μl
  • Anti-HE4 WFDC2 Rabbit Monoclonal Antibody [orb548083]

    ICC,  IF,  IHC,  IP,  WB

    Human

    Rabbit

    Monoclonal

    Unconjugated

    30 μl, 100 μl
  • Anti-HE4/Wfdc2 Antibody [orb546335]

    ELISA

    Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μg, 10 μg