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Catalog Number | orb371697 |
---|---|
Category | Antibodies |
Description | Anti-HE4/WFDC2 Antibody. Tested in ELISA, WB applications. This antibody reacts with Human. |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Immunogen | E. coli-derived human HE4 recombinant protein (Position: E31-F124). Human HE4 shares 40.4% and 68% amino acid (aa) sequence identity with mouse and rat HE4, respectively. |
Antibody Type | Primary Antibody |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 25 kDa |
UniProt ID | Q14508 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | WAP four-disulfide core domain protein 2; Epididym Read more... |
Note | For research use only |
Application notes | ELISA, 0.1-0.5μg/ml, -Western blot, 0.1-0.5μg/ml, Human. Add 0.2ml of distilled water will yield a concentration of 500ug/ml |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of HE4 using anti-HE4 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: 22RV1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HE4 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HE4 at approximately 25 kDa. The expected band size for HE4 is at 13 kDa.
ELISA, IHC, WB | |
Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IP, WB | |
Human, Mouse | |
Rabbit | |
Monoclonal | |
Unconjugated |
ICC, IF, IHC, IP, WB | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |