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Anti-HDAC8 Antibody

Catalog Number: orb865700

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb865700
CategoryAntibodies
DescriptionAnti-HDAC8 Antibody. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, WB
ReactivityHuman, Mouse, Rat
IsotypeRabbit IgG
ImmunogenE.coli-derived human HDAC8 recombinant protein (Position: D29-E347).
Antibody TypePrimary Antibody
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form/AppearanceLyophilized
ConjugationUnconjugated
MW50 kDa
UniProt IDQ9BY41
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesBone morphogenetic protein 15; BMP-15; Growth/diff
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NoteFor research use only
Application notesWestern blot, 0.25-0.5 μg/ml, Mouse, Rat ELISA, 0.1-0.5 μg/ml, -. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml
Expiration Date12 months from date of receipt.
Anti-HDAC8 Antibody

Western blot analysis of HDAC8 using anti-HDAC8 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC8 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HDAC8 at approximately 50 kDa. The expected band size for HDAC8 is at 42 kDa.

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