GRP78 BiP/HSPA5 Rabbit Polyclonal Antibody

SKU: orb76328

Description

GRP78 BiP/HSPA5 Rabbit Polyclonal Antibody

Research Area

Cell Biology, Disease Biomarkers

Images & Validation

• Tested Applications: ICC, IHC, WB
• Dilution Range: Immunocytochemistry, 0.5-1μg/ml, Human, Mouse, Rat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, Human, Mouse Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human GRP78 BiP, identical to the related mouse and rat sequences.
• Target: Endoplasmic reticulum chaperone BiP
• Molecular Weight: 78 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca (2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP; HSPA5; GRP78

IHC analysis of orb76328.

IHC analysis of orb76328.

IHC analysis of orb76328.

Confocal immunofluoroscence analysis of HELA cells using HSPA5 antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded Rat Intestine Tissue using HSPA5 antibody

Western blot analysis of Lane 1: Rat Testis Tissue Lysate, Lane 2: A549 Cell

Quick Database Links

Gene Symbol

Endoplasmic reticulum chaperone BiP

UniProt

P11021