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Anti-Granzyme K/GZMK Antibody
Catalog Number: orb1290011
| Catalog Number | orb1290011 |
|---|---|
| Category | Antibodies |
| Description | Anti-Granzyme K/GZMK Antibody. Tested in IHC applications. This antibody reacts with Human. |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IHC |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human Granzyme K/GZMK, which shares 71.4% and 73.9% amino acid (aa) sequence identity with mouse and rat GZMK, respectively. |
| Antibody Type | Primary Antibody |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Form/Appearance | Lyophilized |
| Conjugation | Unconjugated |
| MW | 41 kDa |
| UniProt ID | P49863 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | ELAV-like protein 2; ELAV-like neuronal protein 1; Read more... |
| Note | For research use only |
| Application notes | Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml |
| Expiration Date | 12 months from date of receipt. |
IHC analysis of Granzyme K/GZMK using anti-Granzyme K/GZMK antibody. Granzyme K/GZMK was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Granzyme K/GZMK Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
