Anti-GPR174 Antibody

• Catalog Number: orb1422042
• Category: Antibodies
• Description: Rabbit polyclonal antibody to GPR174.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• UniProt ID: Q9BXC1
• Tested applications: IF, WB
• Dilution range: WB: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IF: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
• Clone Number: GPR174
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Probable G-protein coupled receptor 174
• Note: For research use only

Immunofluorescent analysis of GPR174 staining in LOVO Cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with Alexa Fluor 647-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Western blot analysis of GPR174 expression in BV2 (A), PC12 (B), A549 (C), Hela (D) whole cell lysates.