GPD1L Rabbit Polyclonal Antibody

SKU: orb546380

Description

Anti-GPD1L Antibody. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Cancer Biology, Cell Biology, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat ELISA, 0.1-0.5μg/ml
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human GPD1L recombinant protein (Position: A19-T351).
• Target: Glycerol-3-phosphate dehydrogenase 1-like protein
• Molecular Weight: 35-40 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
• Concentration: 500 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GPD1 L; GPD1L; KIAA0089

Western blot analysis of GPD1L using anti-GPD1L antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: human placenta issue lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: human Caco-2 whole cell lysates, Lane 8: human K562 whole cell lysates, Lane 9: rat heart issue lysates, Lane 10: mouse RAW246.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPD1L antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GPD1L at approximately 38KD. The expected band size for GPD1L is at 38KD.

Quick Database Links

Gene Symbol

Glycerol-3-phosphate dehydrogenase 1-like protein

UniProt

Q8N335