Anti-GPC3 / Glypican-3 Reference Antibody (codrituzumab)

• Catalog Number: orb1818333
• Category: Antibodies
• Description: Recombinant Antibody
• Clonality: Monoclonal
• Tested applications: ELISA, FC
• Reactivity: Human
• Isotype: IgG1
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Form/Appearance: Purified monoclonal antibody supplied in PBS, pH 6.0, without preservative.This antibody is purified through a protein A column.
• Conjugation: Unconjugated
• MW: 145.5
• UniProt ID: P51654
• Protein Sequence: GPC3 / Glypican-3
• Expression System: CHO Cell
• Endotoxins: < 0.001EU/μg,determined by LAL method.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Glypican-3, GTR2-2, Intestinal protein OCI-5, MXR7
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

The purity of Anti-GPC3 / Glypican-3 Reference Antibody (codrituzumab) is more than 95%, determined by SEC-HPLC.

Immobilized human GPC3, His tag at 2 µg/mL can bind Anti-GPC3 / Glypican-3 Reference Antibody (codrituzumab), EC50=0.008741 µg/mL

Anti-GPC3 / Glypican-3 Reference Antibody (Codrituzumab) -induced ADCC activity was evaluated using human GPC3 CHOS Cell. The max Lysis rate was approximately 30%.

The endocytosis ratio codrituzumab by HuH-7 increased with the increase of antibody concentration, and the Internalization Rate (%) reached 40% at antibody concentration of 1 nM.

Anti-GPC3 / Glypican-3 Reference Antibody (codrituzumab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%

Human GPC3 CHOS cells were stained with Anti-GPC3 / Glypican-3 Reference Antibody (codrituzumab) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC210=2.142 nM