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Glypican 3/GPC3 Antibody

Catalog Number: orb692158

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SizePriceQuantity
100 μg$ 500.00
100 μg Enquire
DispatchCurrently estimated at 1-3 months
Catalog Numberorb692158
CategoryAntibodies
DescriptionAnti-Glypican 3/GPC3 Antibody. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.
ClonalityPolyclonal
Species/HostRabbit
IsotypeRabbit IgG
ConjugationUnconjugated
ReactivityHuman
Form/AppearanceLyophilized
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
PurificationImmunogen affinity purified.
ImmunogenE.coli-derived human Glypican 3/GPC3 recombinant protein (Position: D32-A535).
UniProt IDP51654
MW66 kDa
Tested applicationsELISA, FC, WB
Application notesWestern blot, 0.25-0.5μg/ml Flow Cytometry (Fixed), 1-3μg/1x106 cells ELISA, 0.1-0.5μg/ml. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
Cross ReactivityNo cross-reactivity with other proteins.
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesGlypican-3; GTR2-2; Intestinal protein OCI-5; MXR7
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NoteFor research use only
Expiration Date12 months from date of receipt.
Glypican 3/GPC3 Antibody

Flow Cytometry analysis of HepG2 cells using anti-Glypican 3/GPC3 antibody. Overlay histogram showing HepG2 cells (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Glypican 3/GPC3 Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Glypican 3/GPC3 Antibody

Western blot analysis of Glypican 3/GPC3 using anti-Glypican 3/GPC3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glypican 3/GPC3 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Glypican 3/GPC3 at approximately 66 kDa. The expected band size for Glypican 3/GPC3 is at 66 kDa.

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