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Anti-Ghrelin/GHRL Antibody
Catalog Number: orb1097992
| Catalog Number | orb1097992 |
|---|---|
| Category | Antibodies |
| Description | Anti-Ghrelin/GHRL Antibody. Tested in IHC applications. This antibody reacts with Human. |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Form/Appearance | Lyophilized |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human Ghrelin/GHRL, identical to the related mouse and rat sequences. |
| UniProt ID | Q9UBU3 |
| MW | 20 kDa |
| Tested applications | IHC |
| Application notes | Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Fibroblast growth factor 10; FGF-10; Keratinocyte Read more... |
| Note | For research use only |
IHC analysis of Ghrelin/GHRL using anti-Ghrelin/GHRL antibody. Ghrelin/GHRL was detected in a paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Ghrelin/GHRL Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
